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Micro fluidic chip technology can help medical electronics
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Edit：管理员 Browse：373 Date：2016-03-04
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A hybrid device, which has just been developed, integrates a microfluidic chip for the preparation of a sample and an optical flow control chip for the detection of a single viral RNA molecule. Ebola virus is currently the gold standard relies on the polymerase chain reaction (PCR) this method to amplify the genetic material of the virus for testing. Because PCR on DNA and Ebola virus is an RNA virus, so in the process of PCR amplification and detection of the use of reverse transcriptase enzyme for viral RNA DNA copy.
Scientists at the University of California, Santa Cruz developed a chip based technology, reliable detection of Ebola virus and other pathogenic virus. The system directly to the virus molecule light detection, and can be integrated into a simple and portable instrument for the site to use, because this occasion requires rapid and accurate detection of Ebola virus infection in order to curb the outbreak. The system has two small chips, one is a microfluidic chip, for the preparation of samples, and the other is a light chip, for light detection.
This hybrid device integrated microfluidic chip for sample preparation and used for optical microfluidic chip optical detection of single molecule RNA virus. Microfluidic devices made of silicon polymer - poly two methyl siloxane (PDMS) made of micro channel in the node between the valve and the shipment of samples, to meet the needs of each step of sample preparation. Ebora virus RNA is the target molecule separation method is the corresponding sequence and synthesis of DNA attached to the magnetic particles (called oligonucleotide binding). The collection of magnetic particles with magnets, molecular target is not removed, then heating the target attachment loss, labeled with a fluorescent marker, then pass the light light detection of microfluidic chip.
In laboratory tests, the system can sensitively detect the Ebola virus and would not be mistaken for Sudan Ebola and Marburg virus. Ebora virus test of different concentration, the results show that the six magnitude quantization result is accurate. If the increased during a pre concentration step into the microfluidic chip sample treatment, detection limit will be far more than other chip based method can achieve detection limit, such a detection range of PCR analysis and comparable.
The study published in September 25, 2015 in the "natural" and "science" magazine reported. Senior author, optoelectronics Professor Holger Schmidt, said: "PCR detection than our system is complex, and require a laboratory environment. We can directly detect the nucleic acid, and the detection limit is close to PCR, high specificity. The entire range of clinical concentrations covering the body infected can appear to measure."
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